l-Ornithine:2-Oxoacid Aminotransferase from Squash (Cucurbita pepo, L.) Cotyledons

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منابع مشابه

l-Ornithine:2-Oxoacid Aminotransferase from Squash (Cucurbita pepo, L.) Cotyledons: Purification and Properties.

ORNITHINE: 2-oxoacid aminotransferase (EC 2.6.1.13) has been purified over 400-fold with a total recovery of 14% from acetone powders of cotyledons of germinating squash (Cucurbita pepo, L.) seedlings. The pH optimum of the transamination between l-ornithine and alpha-ketoglutarate is 8 and the Michaelis constants are 4.7 mm and 6.3 mm, respectively. The enzyme has a molecular weight of 48,000 ...

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Activity of b-Galactosidase and Polygalacturonase in Zucchini Squash (Cucurbita pepo L.) Stored at Low Temperatures

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Some Physical Properties of Pumpkin (Cucurbita pepo L. ) and Watermelon (Citrullus lanatus L.) Seeds

Some physical properties of pumpkin (Cucurbita pepo L.) and watermelon (Citrullus lanatus L.) seeds were determined at a moisture content of 9.87% and 2.87% (dry basis), respectively. The mean values of length, width, thickness and geometric mean diameter were 19.92, 11.30, 3.22, 9.71 mm for pumpkin seeds, and 13.28, 8.00, 2.64, 6.52 mm for watermelon seeds, respectively. The mean 1000 seed mas...

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Isolation and Purification of Nuclear Dna from Excised Cotyledons of Cucurbita Pepo

High molecular weight genomic DNA was isolated from excised marrow cotyledons (Cucurbita pepo L., zucchini) using high concentration of ethidium bromide (400 μg.ml–1) in the grinding buffer and phenol deproteinization of the crude nuclear pellet. This preparation of DNA was free of proteins but contained polysaccharide contaminations which did not allow complete digestion with a number of restr...

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Spectral Characterization and Proteolytic Mapping of Native 120-Kilodalton Phytochrome from Cucurbita pepo L.

A spectral, immunochemical, and proteolytic characterization of native 120-kilodalton (kD) phytochrome from Cucurbita pepo L. is presented and compared with that previously reported for native 124-kD phytochrome from Avena sativa. The molecule was partially purified ( approximately 200-fold) in the phytochrome-far red-absorbing form (Pfr) in the presence of the protease inhibitor, phenylmethyls...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1975

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.55.3.502